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Thin Layer Chromatography

Thin layer chromatography (TLC) is used for identifying the compounds, finding out their purity and optimizing the solvent by separating the desired compound. To carry out the separation using this TLC technique, an adsorbent in the powdered form is used as a thin layer. Here, stationary phase consists of solid adsorbent such as silica or alumina coated on glass, plastic support, whereas the mobile phase consists of a solvent or mixture of the solvents. This material is then applied on the glass plate, aluminum foil or plastic sheet or plate.

Thin Layer Chromatography

The chromatographic process completely depends on the type of adsorbent used, its adsorption capacity and the nature of solvents used. Silica gel, alumina and cellulose can be used as the adsorbents. Usually, silica gel is preferred than other adsorbents to get easy, simple and quick results. TLC is commonly used in organic chemistry where there is a need to identify the compounds present in the mixture or solvent and also to get the particular compound separated from the mixture. No matter how many components are present in a mixture, TLC has the power to support the identity of a compound.

This process is much faster as compared to column chromatography. In thin layer chromatography, the solvent with high polarity tends to move all components in a mixture faster, while in lowering the polarity the movement tends to slow down. It is required to get the ideal solvent system to obtain the best possible desired separation. It also allows you to change the chromatography conditions to enhance the resolution of the particular component. The main purpose of using TLC is to get well-defined and well-separated component.

Silica Gel Grade sizes suitable for TLC

  • Silica Gel G
  • Silica Gel H
  • Silica Gel GF254
  • Silica Gel HF254

Swambe Chemicals is specialized in offering quality and effective range of adsorbents that can be used in range of industries and their applications. Silica gel in different grade sizes with and without binder is available to get the high performance thin layer chromatography. We are helping our clients across the globe by providing the right solution for the desired separation process.